Retinal Pigment Epithelial Cell Conditioned Medium Enhances the Yield of RPE Cells Differentiated from Human Embryonic Stem Cells
نویسندگان
چکیده
منابع مشابه
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
We describe a robust method to direct the differentiation of pluripotent stem cells into retinal pigment epithelial cells (RPE). The purpose of providing a detailed and thorough protocol is to clearly demonstrate each step and to make this readily available to researchers in the field. This protocol results in a homogenous layer of RPE with minimal or no manual dissection needed. The method pre...
متن کاملIn vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells.
PURPOSE To determine whether primate embryonic stem (ES) cell-derived pigment epithelial cells (ESPEs) have the properties and functions of retinal pigment epithelial (RPE) cells in vitro and in vivo. METHODS Cynomolgus monkey ES cells were induced to differentiate into pigment epithelial cells by coculturing them with PA6 stromal cells in a differentiating medium. The expanded, single-layer ...
متن کاملAutophagy Regulates Proteasome Inhibitor-Induced Pigmentation in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells
The impairment of autophagic and proteasomal cleansing together with changes in pigmentation has been documented in retinal pigment epithelial (RPE) cell degeneration. However, the function and co-operation of these mechanisms in melanosome-containing RPE cells is still unclear. We show that inhibition of proteasomal degradation with MG-132 or autophagy with bafilomycin A1 increased the accumul...
متن کاملSubretinal transplantation of putative retinal pigment epithelial cells derived from human embryonic stem cells in rat retinal degeneration model
OBJECTIVE To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases. METHODS The embryoid bodies were formed from hESCs and attached on the matrigel coated culture dishes. The neural structures consisting neural precursors were sel...
متن کاملMorphological changes in injured retinal pigment epithelium and photoreceptor cells after transplantation of stem cells into subretinal space
Introduction: Degenerative retinal diseases are main cause of irreversible blindness. Stem cells therapy is a promising way in these diseases. Therefore, mesenchymal stem cells because of its safety can produce degenerated cells and can play important role in treatment. The aim of this study was to examine morphological changes in injured retinal pigment epithelium (RPE) and photoreceptor cells...
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ژورنال
عنوان ژورنال: Journal of Clinical & Experimental Pathology
سال: 2015
ISSN: 2161-0681
DOI: 10.4172/2161-0681.1000216